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Beer’s law states that A = abc, where A is absorbance, a is the molar absorptivity constant, b is the path length, and c is the molar concentration. Which of the following would be an incorrect application of Beer’s law?

(A) The path length is irrelevant, as long as it is inversely proportional to molar concentration when determining the concentration of an unknown compared to a standard curve.
(B) The molar concentration is directly related to the absorption of light by the sample and is used to develop a standard curve.
(C) The concentration of an unknown sample can be easily determined from a standard curve of known concentration plotted against absorbance for a fixed path length.
(D) The use of Beer’s law and a spectrophotometer for a colored sample is a better method of determining molar concentration than visual comparison.

Aniket Singh , 10 Months ago
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anser 1 Answers
Askiitians Tutor Team

The statement that would be an incorrect application of Beer’s law is:

(A) The path length is irrelevant, as long as it is inversely proportional to molar concentration when determining the concentration of unknown as compared to a standard curve. This statement is incorrect because, according to Beer’s law (A=abc) , the path length (b) is an important factor and must remain constant when determining the concentration of an unknown sample using a standard curve. The path length should not be inversely proportional to the molar concentration; instead, it should be fixed to ensure accurate and consistent measurements. Beer’s law assumes that the path length through the sample is constant, which allows the absorbance to be directly proportional to the molar concentration.





Last Activity: 10 Months ago
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