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For cloning Dolly enucleated egg cell was obtained from _________________.\tHolstein Friesen\tFinn Dorset Sheep\tScottish blackface ewe\tApis cerana indica

Debartha Paul , 10 Years ago
Grade 12th Pass
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Raheema Javed

Last Activity: 10 Years ago

For cloning Dolly enucleated egg cell was obtained from (3) Scottish blackface ewe.

To produce Dolly, scientists used an udder cell from a six-year-old Finn Dorset white sheep. They had to find a way to 'reprogram' the udder cells - to keep them alive but stop them growing – which they achieved by altering the growth medium (the ‘soup’ in which the cells were kept alive). Then they injected the cell into an unfertilised egg cell which had had its nucleus removed, and made the cells fuse by using electrical pulses. The unfertilised egg cell came from a Scottish Blackface ewe. When the research team had managed to fuse the nucleus from the adult white sheep cell with the egg cell from the black-faced sheep, they needed to make sure that the resulting cell would develop into an embryo. They cultured it for six or seven days to see if it divided and developed normally, before implanting it into a surrogate mother, another Scottish Blackface ewe. Dolly had a white face.

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