explain sepration and isolation of dnafragment

Hello Stident
Separation and isolation of DNA fragment are follows;

• Cutting of DNA by restriction endonuclease results in DNA fragments
• The fragments are separated by gel electrophoresis
• the commonly used gel is Agarose gel, anatural polymer found in sea weeds.
• Agarose gel provides a sieving effect means separate according to their size,smaller fragment,farther it moves.
• After that these are stained withEthidium Bromide followed by UV exposure.
• Finally Elution occurs in which separated bands of DNA are cut from the agarose gel.